Kris Budd #IAmScience

Kris Budd

Kris Budd, a Ph.D. candidate in Lori Eggert’s lab, works with Bond LSC to track elephant DNA in Southeast Asia. | photo by Allison Scott, Bond LSC

“#IAmScience because I have the ability to transform the fate of endangered species.”

If someone had told Kris Budd that she’d be investigating elephant feces on daily basis in her Ph.D. program, she wouldn’t have bought it. If they’d said she’d realize it’s a passion of hers, she would’ve been in shock.

As a third year Ph.D. candidate in Lori Eggert’s lab, Budd is able to do meaningful work that is helping endangered elephants through feces.

“I always wanted to work with an endangered species,” Budd said. “I’ve always been excited about this, and the more you find out about elephants, the more you love them.”

Budd receives samples of elephant dung from Southeast Asia, and singles out the elephant DNA from the other species present in the sample — most commonly microbes and insects, but sometimes even goats and humans.

“You have to be aware that there can be DNA from anything that walked by that day,” Budd said. “We have to use specific tools to make sure we just get the elephant DNA.”

“After processing, we use the DNA Core Facility in Bond LSC, which isn’t an easy task since we typically run hundreds of samples several times for a single study.” Budd said. “But they always do it with grace.”

Budd then uncovers the genetic makeup with the help of the Informatics Research Core Facility (IRCF) and meetings with members of Chris Pires’ lab in Bond LSC.

From there, Budd is able to input the DNA into a system and keep track of the elephants overseas. They, however, become way more than numbers to her.

“They might just be a sample in your data, but you get to know the elephants,” Budd said. “Elephants are so much more. There are so many different elements to them — genetics, behavior, and ecology. Their evolutionary history and family structure tells a story, all seen through feces.”

Her work is about to get a lot more hands-on next semester, as Budd will be traveling overseas to collect samples herself.

“We’re going in the spring and we’ll teach local technicians how to go about collecting samples,” Budd said. “We’ll collect a lot of samples of our own while we’re there, too.”

Budd will be able to determine what is happening with the elephant populations in Southeast Asia more closely then.

“There’s a big push with critically endangered species to translate data into something that can help them,” Budd said. “The samples I’m working on from Myanmar are actually an extinct population, but we want to re-wild similar elephants in the same location.”

Essentially, that would require Budd to find a genetically similar elephant population that would be most likely to thrive in the same environment.

While actually implementing the re-wild process won’t happen for a while, Budd is certain about the influence of Bond LSC in the elephant re-wilding’s future success.

“My work wouldn’t be possible without the people who work at Bond LSC,” Budd said.

A zebrafish’s empty stomach can help scientists understand brain function

A new test can show how much a zebrafish larva has eaten. This basic information could be crucial to upcoming discoveries.

A zebrafish swims in its tank. Understanding how zebrafish move  can give researchers insight into how certain diseases impact human motion.  Photo courtesy National Institute of Child Health and Human Development.

A zebrafish swims in its tank. Understanding how zebrafish move can give researchers insight into how certain diseases impact human motion. Photo courtesy National Institute of Child Health and Human Development.

By Eleanor C. Hasenbeck | Bond Life Sciences

Until now, it was hard to know when a zebrafish larva had a full stomach.

Researchers in Anand Chandrasekhar’s Lab at the Bond Life Sciences Center are studying the networks of neurons that control the zebrafish’s jaw, but to do that, they first had to figure out just how much these fish larvae eat.

They didn’t just need to understand it, they had to be able to test it. The successful development of a test that measures how well a zebrafish larva can eat has already lead to more discoveries.

To develop this test, researchers fed the larvae fluorescent fish food for three hours at a time. That’s just long enough for them to eat it, and just before their intestine starts to push it out. Their tiny, fluorescent bellies were examined under a microscope and scored. A zero meant that there was no food in the larva’s stomach, while a three meant the stomach was completely full.

With this feeding test under their belts, researchers are now able to form and test more research questions. The Chandrasekhar Lab used it to better understand networks of branchiomotor neurons, the circuitry that controls jaw movement. These neurons also control the gill muscles that move in automatic movements, such as breathing, just as neurons in the human brainstem allow us to breathe without thinking. Researchers look at the zebrafish’s very basic motor neurons to understand how these nerves develop, heal and control simple tasks.

One experiment tested how fewer branchiomotor neurons affect the action of eating. They destroyed 50-80 percent of the larvae’s branchiomotor neurons using a chemical-genetic method. The fish with fewer branchiomotor neurons also ate less food. They tried a similar experiment, but this time using lasers to remove only a select portion of branchiomotor neurons that controls a set of jaw muscles. Again, they found that the larvae that went under the laser were not able to eat as much food as the normal larvae. Finally, they conducted the food-intake test with mutant larvae that did not have any cranial motor neurons. As the researchers predicted, the mutants were not able to eat.

Now, they want to test how these animals eat when these neurons develop differently. While the first tests essentially smashed nearly all of the zebrafish’s branchiomotor neuron circuitry, their upcoming research will examine what happens when these circuits are wired in a different way, with neurons in the wrong spots. They’ll be looking at much subtler changes, Chandrasekhar said.

“If they don’t eat properly, do they move their jaw properly? That’s the next question that I want to answer,” said Emilia Asante, a doctoral candidate in the lab. “Are the axons not going to the right position? Are their neuromuscular junctions not properly positioned? Are there fewer of them in the mutant? There are all these questions that these assays are actually critical in answering.”

Asante is also working to make the feeding test more quantitative and less labor intensive. In its current form, someone has to look at each larva and judge if the food in its stomach makes it a zero, one, two or three. They want to be able to measure more accurately how much food is in the fish’s belly. If they were able to develop a faster and more accurate test, researchers would be able to rapidly measure food intake in a greater number of fish and to test the effects of many different chemical and environmental factors.

Zebrafish are a unique lab animal model used in research for a number of reasons. They’re easy to observe because the embryo develops quickly in an egg outside of the mother. They’re transparent, so researchers can make certain cells visible using florescence and observe them in a developing animal without killing it. Their genome is fully sequenced, so researchers can easily create mutations in specific genes using CRISPR technology.

“Many of the same circuitry that you find in humans are also there in more primitive organisms, and one of them happens to be the zebrafish,” Chandrasekhar said. “It has got some of the same types of neurons and the same types of circuits that you can find in humans.”

Research into the zebrafish’s neural networks can help researchers understand diseases like Amyotrophic Lateral Sclerosis, better known as Lou Gehrig’s disease, which causes a loss of function in a human’s motor abilities, including those of the branchiomotor neurons.

This study “Role of branchiomotor neurons in controlling food intake of zebrafish larvae” was recently published in the Journal of Neurogenetics.

Lisa Caesar #IAmScience

Lisa Caesar

Lisa Caesar is a biological sciences Ph.D. candidate who works in Laura Schulz’s lab. | photo by Allison Scott, Bond LSC

 

“#IAmScience because education and my pursuit of learning became my ticket out of poverty and a way that I can really help others.”

“Mother knows best” rings true for Gerialisa Caesar. In her family, the career options were either to be a lawyer, engineer or doctor.

But with little interest in law and a greater love for science than math, Caesar decided to pursue her doctorate.

“I graduated high school at 16 fully equipped to enter the work force,” Caesar said. “I went back to school to study science because my mom basically told me I had to, but biology fit me really well, so it worked out.”

Initially, Caesar was hesitant that she could even become a scientist.

“I had an idea of what a scientist was, and it wasn’t me,” Caesar said. “After I immigrated from Guyana, my undergraduate advisors, Drs. Carroll and Catapane, explained the opportunities I had to study biology. It opened the world of possibilities for me to pursue science.”

Now, as a biological sciences Ph.D. candidate in Laura Schulz’s lab, Caesar focuses on women’s health and reproductive biology.

“My research is to understand how a mother’s nutrition prior to and during pregnancy affects the baby’s development,” Caesar said. “This is important as several studies link maternal diet during pregnancy to an increase in susceptibility of the offspring developing diseases such as obesity, diabetes and hypertension in adulthood.”

That includes connecting how mother’s diets cause fetal health issues, which is a highly debated topic.

“For example, a lot of women are told to take folic acid pills during pregnancy,” Caesar said. “Doing that has been tied to help with neural development concerns — things like spina bifada [a birth defect that affects developing babies’ spinal cords]. Understanding the mechanisms that make that occur would aid in developing better interventions to prevent such occurrences.”

As a result, Caesar’s research has the ability to help the world’s greatest health concerns in an impactful way.

“My work is directly contributing to prevent health issues that exist for future generations,” Caesar said. “Knowing that is motivating.”

After she earns her Ph.D., Caesar would love to work for the Center for Disease Control and Prevention.

“The CDC has immediate response teams that react to major disease concerns. Being a part of that would be incredible,” Caesar said. “There’s something about working for the CDC and being able to have a meaningful impact within the community that calls me.”

Caesar plans to channel that passion regardless of where she ends up, though.

“Biology is literally the study of life,” Caesar said. “As much as I thought science was challenging, I am able to see how it affects everyday life. I have a purpose, and I know what I do is going to make a difference.”

Dr. Burke wins Distinguished Professor Award

Donald Burke-Agüero

Donald Burke-Agüero stands in his office in Bond LSC, holding a model of an RNA protein structure. Burke-Agüero studies the bio-chemical functions of RNA, and how those functions might be able to be artificially designed or replicated. | Phillip Sitter, Bond LSC

By Samantha Kummerer, Bond LSC

“He’s a triple threat in science,” Bond Life Sciences researcher David Pintel said.

Donald Burke’s combination of scientific excellence, caring mentorship and devotion to the University of Missouri led Pintel to nominate Burke for the 2017 William H. Byler Distinguished Professor Award.

The university agreed, awarding him the honor in October.

The University-wide accolade recognizes a faculty member for their “outstanding abilities, performance, and character.” When Pintel read this description he said he instantly thought of Burke.

This is the first time a faculty member from Bond LSC has won the award. Pintel said the award shows the quality of people here and the department is proud of him for receiving it.

Burke is a Molecular Microbiology & Immunology professor. His lab in Bond LSC studies RNA, the molecule used to help cells copy genetic information from DNA.

For Burke, the award is an honor.

“You always try to do everything you can to contribute to making the place great and you don’t do it for the purpose of being recognized, yet when the recognition happens, it’s nice,” he said.

Burke’s lab hosts students from the high-school to post-doctoral level. For each member, Burke said he tries to figure out their motivation. He starts by just asking questions and is able to align their goals and his.

“You can’t just say ‘This is what we do in my lab, deal with it.’ You can’t do that,” Burke elaborated, “Yes, it’s a lot of work and a lot of effort, but it has ceased being a burden for me. Now, I think of it as a new fun challenge.”

He said that challenge has paid off because every student that has gone through his lab has taken his science in new directions.

Pintel said this connection Burke has with his students is easy to see.

“People in his lab love him and, to me, that’s always a good sign,” Pintel said. “When I see people in a lab like that, I know it’s a good environment. That always means something good is going on.”

During their time in his lab, Burke tries to teach his students the importance of working as a team as well as learning understand the research process.

“Some of the things I’m most proud of aren’t necessarily the most impactful research, but the things where I know the struggle that went into solving the puzzle that was in front of us,” he explained. “The thrill of the chase may not be as interesting to the rest of the world but you know how hard that person worked and how many false leads there were.”

Recently, Burke has expanded his passion for motivating others to a new area. In July, Burke became the Interim Chair of Molecular Microbiology & Immunology.

“Now that he’s the interim chair he is trying to provide the same kind of supportive environment for the department that he’s been doing for his lab,” Pintel said.

Burk said he wants to help other faculty members achieve their goals and prioritize tasks. He is also involved in a new faculty search where he works to ensure the applicant pool reflects those that might be wanting to apply by removing language that may prevent people from applying.

“Again, I want to help find what obstacles can be in their way and get rid of those before it slows them down,” Burke said restating his passion for helping others achieve excellence.

Donald Burke-Aguero is an MU professor in the Department of Molecular Microbiology & Immunology. He received his bachelors’ degree from the University of Kansas and his Ph.D. from the University of California, Berkeley. Burke joined the MU community in 2005.

Julia Brose #IAmScience

Julia Brose

Julia Brose is a senior studying biochemistry at Mizzou. She works in Chris Pires lab in Bond LSC. | Photo by Allison Scott, Bond LSC

“#IAmScience because I really enjoy discovering and being around people who cultivate a positive learning environment.”

As a freshman at Mizzou four years ago, Julia Brose knew she had a love for plants. That, however, competed with her fascination with biochemistry.

Luckily, she found and was selected for FRIPS, Freshman Research in Plant Sciences, which allowed her to do both. The program is made up of 10 freshman each year who gain valuable hands-on research experience with plants.

“I started working in Bond LSC, and that’s really where I found my love for research,” Brose said. “Working with plants allowed me to explore that interest, while still majoring in biochemistry. I have the best of both worlds.”

Her degree in biochemistry coupled with her research experience has given her a number of unique opportunities. One of which was being a Cherng Summer Scholar at Bond LSC last summer where she studied plants and their protein makeup.

“I was looking at amino acids, which are the building blocks of proteins in plants,” Brose said. “Specifically, I was looking for the content in seeds within Brassica, a species that includes cauliflower and kale.”

Another unique opportunity Brose earned was in summer of 2016. She worked at Stanford University as part of a fellowship for the American Society of Plant Biologists.

“I studied novel plant defense compounds —how plants protect themselves,” Brose said. “People can move around and gain protection that way, but plants need different chemicals to protect themselves.”

Her background in biochemistry and her experience with plant research at Bond LSC in Chris Pires’ lab provided her with the ability to analyze the defense structures in a unique way. As a result, she uncovered something that others had either ignored or overlooked.

“I found that the chemicals we see in leaves are in roots, too,” Brose said. “No one else had looked there before, so it was cool to be the first.”

Her work at Stanford led them to send her to a conference in Hawaii earlier this year. While there, Brose was able to present a poster on her findings as well as network with a number of successful scientists in a variety of fields.

As she nears graduation this spring, Brose has begun looking into graduate school options. Those are largely based upon the experience she had in Hawaii.

“I was able to make connections that have influenced my plans for the future as far as where I’m applying to graduate school,” Brose said.

Wherever she ends up, Brose hopes to teach.

“I like mentoring students and being around a learning environment,” Brose said. “That is really fostered in a university.”

Marianne Emery #IAmScience

Marianne Emery

Marianne Emery is a Ph.D. candidate in Ruthie Angelovici’s Lab in Bond LSC. | photo by Allison Scott, Bond LSC

“#IAmScience because it leads to innovation that makes for a better world, which is an awesome thing to be a part of.”

It’s good to have a role model, and Marianne Emery has always looked up to female pioneer scientists.

One of her favorites is Barbara McClintock, a Nobel prize winning botantist who studied how the chromosomes of corn change during reproduction.

It is from women like McClintock that Emery is encouraged to always be impactful with her research and overcome obstacles with grace.

“I think it’s inspiring to see these women in positions that have typically been male-dominated,” Emery said. “You lose your confidence sometimes when things just don’t work. You’re continuously met with obstacles, but you have to keep going.”

And that she has.

Emery works in Ruthie Angelovici’s lab at Bond LSC to understand what controls protein levels in seed. She primarily spends her time on the computer working with large data sets and trying new software, but is always excited about the findings she’s able to uncover.

“I enjoy developing new skill sets every day,” Emery said. “The most important thing I’ve learned so far is how to communicate my science and how to communicate when I’m having an issue. Conveying a problem and problem-solving in general can be hard.”

Still, Emery continues to focus on improving on a daily basis. She hopes to work for a company like Monsanto after earning her Ph.D.

“I really like the business side of science,” Emery said. “Ultimately, a bigger company would be the best fit. I also really like policy and the patenting process.”

Wherever Emery ends up, though, she hopes to become like the women who pioneered science.

“Female scientists have been so inspiring to me,” Emery said. “I hope that one day I can be a leader and a role model for other young women who aspire to be involved with science.”

The Truth Behind BPA- Free Labels

Cheryl Rosenfeld

Cheryl Rosenfeld, a Bond Life Sciences Center investigator and professor of biomedical sciences at the University of Missouri. | photo by Jinghong Chen, Bond LSC

What companies aren’t telling you about their merchandise

By: Samantha Kummerer, Bond LSC

Bisphenol A, otherwise known as BPA, is used to make plastic containers, coats the inside your metal food cans, and leaches into your food and water.

BPA has concerned scientists, health practitioners and the general public for many years because of its potential to mimic hormones and disrupt the developmental stages in animals.

Opposition to the chemical has led to certain manufacture’s marketing their items, such as food containers and water bottles, as“BPA–Free” and a global push to reduce the usage of BPA in commonly used household-items.

However, new evidence suggests consumers may not be as ‘free’ from harm as they think.

Bond Life Sciences Center researcher Cheryl Rosenfeld suggested that many consumers might not be aware of how these alternatives are made. BPA-substitutes — bisphenol S (BPS), bisphenol F (BPF) and bisphenol AF (BPAF) — aren’t too different from BPA chemically.

“They’re just playing with various synthetic structures is what many industrials groups are doing,” she said.

BPA is made up of two chemical compound groups called phenols. Picture two rings with different elements connected. In BPF, BPS and BPAF all those same parts are still present, and the only change is that the rings are rotated differently and contain various branching chemicals .

Since these alternate chemicals are structurally similar to BPA, they still bind to estrogen receptors, receptors to which the natural hormone estrogen binds and activates, which results in up- or down-regulation in the expression of various genes.

“The worst problem is that humans and animals are unknowingly serving as test subjects. Industrial companies are not required to show definitive evidence that these alternatives to BPA are safe. They can cite their own limited studies, but currently, no rigorous testing is required for these BPA-alternative chemicals that are being to flood the market. There is no requirement for the products that contain these chemicals to state as such. Thus, consumers cannot make educated decisions when purchasing various food and beverage products,” Rosenfeld said speaking of the industry.

Substitutes aren’t always better

Rosenfeld recently published a literature review exploring the use of the BPA-alternatives and their potential risk. She began the work out of curiosity since her Bond LSC lab studies the harmful effects of BPA.

After putting the puzzle pieces together, the big picture began to emerge that exposure of rodent models to these substitute chemicals exert analogous effects as BPA — depressive behaviors, increased anxiety, decrease in social behavior and decreased maternal/paternal care. In some cases, the alternatives have an even greater effect, according to Rosenfeld.

Understanding BPA

Production of the industrial chemical, BPA, began in the mid 1900’s to make plastics. Today, it can be found in everything from water bottles to storage containers and even food. With this increase exposure comes an increased risk to consumers.

Past studies linked exposure to the chemical with health effects on the brain and behavior and many other widespread effects.

When BPA enters the body, the can at least partially metabolize and break down the chemical via enzymes, which is then removed from the body. But there is a limited supply of such metabolizing enzymes used and those consuming BPA daily will end up overwhelming their body’s ability to metabolize and eliminate it with the net effect that BPA can accumulate over time and continue to exert potential harmful effects.

The most serious effect of BPA and now the substitutes happens during development. Fetuses have poorability to break down the chemical. Evidence links BPA exposure during development with neurological disorders like autism spectrum disorders (ASD) and attention deficit hyperactivity disorder (ADHD).

Most studies have examined the effects of BPA alternatives in rodents and zebrafish. While numerous factors prevent scientists from establishing causation in humans, Rosenfeld suggested it is time to at least start correlating early life exposure to BPA alternatives and risk of neurobehavioral disorders, including ASD, ADHD, and other neurodegenerative disorders.

“What’s worrisome is the women who are seeking to become pregnant or are currently pregnant, they are under the impression that they are making positive choices for their sons and daughters by using BPA-free products, but such products likely contain these BPA-substitute chemicals that can result in equal and possibly even greater negative consequences for their unborn offspring. Ultimately, the question is then what sort of products should be using?” Rosenfeld explained.

Humans aren’t alone in the impact. Rosenfeld explained these chemicals don’t break down in the environment and could play a part in the decline of certain animals. Previous studies by Rosenfeld’s lab and others at MU revealed BPA has the ability to feminize what should otherwise be male turtles.

“I just wish we could stop and pause and think about the havoc we are wrecking on our own environment and ourselves,” she said.

Without definitive results, a lot of unanswered questions remain. Future studies will likely explore the full range of effects caused by the BPA alternatives. Rosenfeld is calling for researchers to begin to treat these alternatives like BPA and put them through rigorous studies. These studies, however, come with a cost.

Rosenfeld’s lab is funded for BPA research. To add these additional chemicals to her current studies, the cost would double and triple due to the increased amount of animal test subjects that would be required.

“They keep upping the bar of how many replicas we have to test. If you want to show effects, if you want us to believe your results, we have to test 12-15 animals, well then you need to test another 12-15 animals in these groups and then your research dollars are all-of-a-sudden gone,” Rosenfeld said.

Research costs are going up and in the meantime BPA is still being produced in high quantities, so the industry as a whole has to decide where to allocate its funds.

“It’s a moving target. We’re trying to keep up and they keep synthesizing more and more chemicals everyday. The problem is we are completely outspent in terms of avaibable research dollars compared to the money industrial companies have on hand to fight tighter reguation. It’s not even a fair fight,” she said.

Rosenfeld hopes this new publication prompts scientists and the public to begin to question and call for more action on testing these pervasive BPA subsitutes. The silver lining is even though we can’t control our exposure to these chemicals, Rosenfeld hopes we might be able to find a way to combat our exposure.

Cheryl Rosenfeld is a Biomedical Sciences professor,a researcher in Bond Life Sciences Center, and research faculty member in the Thompson Center for Autism and Neurobehavioral Disorders. Rosenfeld specializes in how the early in utero environment can shape later offspring health, otherwise considered developmental origins of health and disease (DOHaD)She earned her bachelors and doctor of veterinary medicine degrees from the University of Illinois and her PhD from the University of Missouri.